DEVELOPMENT OF DOT-ELISA FOR DETECTION OF HAEMONCHUS-CONTORTUS ANTIGEN

Haemonchosis caused by Haemonchus contortus (Nematoda: T r i c h o s t r o n g y l i d a e) is one of the major health hazards, affecting th e sheep/goat industry. Early diagnosis, followed by treatment is essen tial to control this disease. Hence, in the present studies, an attemp t has been made to develop Dot-ELTSA for the detection of H. contortus soluble antigen. The test involves competition between the free antig en and solid phase bound antigen for anti-H. contortus antibodies. The test was able to detect as low as 10 pg of the soluble antigen. Poten tial applications of the test after testing in clinical samples are di scussed.