MOLECULAR HAPLOTYPING OF GENETIC-MARKERS 10 KB APART BY ALLELE-SPECIFIC LONG-RANGE PCR

Haplotypes, combinations of polymorphic markers in a chromosome, are c ritical for genome diversity research, However, their utility in popul ation samplings is compromised by uncertain linkage phase determinatio ns from unrelated individuals, Molecular haplotyping accomplishes dire ct phase determination by generation of hemizygous templates from dipl oid genomic samples, We report molecular haplotyping by allele-specifi c long-range PCR of two markers 9.5 kb apart at the CD4 locus: a bi-al lelic Alu deletion and a multi-allelic repeat. We verified CD4 molecul ar haplotypes by classical Mendelian analysis, Molecular haplotyping s hould prove useful in mapping disease genes and in establishing founde r effects.