Jp. Hoover et al., COMPARISON OF VISUAL INTERPRETATIONS AND OPTICAL-DENSITY MEASUREMENTSOF 2 ANTIGEN TESTS FOR HEARTWORM INFECTIONS IN DOGS, Canine practice, 21(3), 1996, pp. 12-20
Sera from 61 dogs were tested for Dirofilaria immitis antigens by two
antigen tests using visual interpretations and a microwell optical den
sity (OD) reader. Dogs were necropsied to determine if they were infec
ted and to recover all worms. Investigation of test kit OD readings re
vealed major sources of variability existed in setting up the two test
s. This report presents our findings and compares the subjective visua
l interpretations with the objective optical density values for D. imm
itis antigens in dogs by the two tests including an assessment of the
dependability of these tests in predicting worm burdens of infected do
gs.