A COST-EFFECTIVE BASE-MATCHING ASSAY WITH LOW BACKGROUNDS

Authors
SU X MUSHINSKY G COMEAU AM
Citation
X. Su et al., A COST-EFFECTIVE BASE-MATCHING ASSAY WITH LOW BACKGROUNDS, Nucleic acids research, 24(22), 1996, pp. 4596-4597
Citations number
7
Categorie Soggetti
Biology
Journal title
Nucleic acids research → ACNP
ISSN journal
03051048
Volume
24
Issue
22
Year of publication
1996
Pages
4596 - 4597
Database
ISI
SICI code
0305-1048(1996)24:22<4596:ACBAWL>2.0.ZU;2-7
Abstract
Base-matching or so-called mini-sequencing is a powerful technique for genotyping and mutation identification, However, its application is o ften hampered by high background and high cost. We have decreased the background by similar to 5-fold by incorporating an end-blocking step and using only 1/10 of the usual nucleotide concentrations.