FLUORESCENT LOCATION OF CELLS OF CYTOLOGICAL INTEREST IN CERVICAL SMEARS PRESTAINED WITH THIONIN

Authors
STEVEN FS DESAI M SIN J PALCIC B
Citation
Fs. Steven et al., FLUORESCENT LOCATION OF CELLS OF CYTOLOGICAL INTEREST IN CERVICAL SMEARS PRESTAINED WITH THIONIN, Anticancer research, 16(3A), 1996, pp. 1193-1196
Citations number
10
Categorie Soggetti
Oncology
Journal title
Anticancer research → ACNP
ISSN journal
02507005
Volume
16
Issue
3A
Year of publication
1996
Pages
1193 - 1196
Database
ISI
SICI code
0250-7005(1996)16:3A<1193:FLOCOC>2.0.ZU;2-U
Abstract
Cervical cells of cytological interest were located in conventional sm ears, treated with thionin for quantitative DNA staining by subsequent treatment with fluorescent probes for a cell surface protease. Normal mature cervical epithelial cells failed to bind these fluorescent pro bes whilst metaplastic cells, glandular. cells, and dyskaryotic cells were readily located By this means, the nuclear staining of these fluo rescent cells of cytological interest enabled them to be classified by a cytologist.