HPLC DETERMINATION OF TRYPTOPHAN ENANTIOMERS WITH PHOTOMETRIC, FLUOROMETRIC AND DIODE-LASER POLARIMETRIC DETECTION

The combined utilization of photometric, fluorimetric and polarimetric detection liquid chromatography detectors in series for the identific ation and quantification of D-tryptophan and L-tryptophan was evaluate d. Detection limits of about 1 mu g were established with the range of linearity extending to about 100 mu g. The relative standard deviatio n of the D-form and L-form tryptophan were 7.32 and 4.22 %, respective ly. The amount unknown of tryptophan enantiomers in the different mixt ures was determined with an accuracy of 1.58% at the 40 mu g injection level.