1,1'-[ETHENYLIDENEBIS(SULFONYL)]BIS-BENZENE - A USEFUL PRE-CHROMATOGRAPHIC DERIVATIZATION REAGENT FOR HPLC ANALYSES OF THIOL DRUGS

Authors
CAVRINI V GOTTI R ANDRISANO V GATTI R
Citation
V. Cavrini et al., 1,1'-[ETHENYLIDENEBIS(SULFONYL)]BIS-BENZENE - A USEFUL PRE-CHROMATOGRAPHIC DERIVATIZATION REAGENT FOR HPLC ANALYSES OF THIOL DRUGS, Chromatographia, 42(9-10), 1996, pp. 515-520
Citations number
19
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Chromatographia → ACNP
ISSN journal
00095893
Volume
42
Issue
9-10
Year of publication
1996
Pages
515 - 520
Database
ISI
SICI code
0009-5893(1996)42:9-10<515:1-AUP>2.0.ZU;2-X
Abstract
The use of 1,1'-[ethenylidenebis(sulfonyl)]bis-benzene as a pre-chroma tographic reagent for LC analysis of thiol drugs is proposed. The reag ent reacts rapidly (2 min) under mild conditions (pH 7.5, ambient) wit h thiol drugs and the resulting adducts can be chromatographed under r eversed-phase conditions (C-18 column). Excess reagent can be removed from the reaction mixture by simple liquid-liquid extraction with chlo roform. The HPLC method using UV detection was successfully applied to the analysis of reduced glutathione (GSH), N-acetylcysteine, N-2-merc aptopropionylglycine and captopril in their commercial formulations.