PURIFICATION AND CHARACTERIZATION OF A 93 KDA ASPERGILLUS-FUMIGATUS ANTIGEN WITH DIAGNOSTIC POTENTIAL

Authors
HAYNES KA TUINSTRA P HUGHES TA WIJNANDS LM ROGERS TR ALLEN AK
Citation
Ka. Haynes et al., PURIFICATION AND CHARACTERIZATION OF A 93 KDA ASPERGILLUS-FUMIGATUS ANTIGEN WITH DIAGNOSTIC POTENTIAL, Journal of medical and veterinary mycology, 34(6), 1996, pp. 421-426
Citations number
27
Categorie Soggetti
Mycology
Journal title
Journal of medical and veterinary mycology → ACNP
ISSN journal
02681218
Volume
34
Issue
6
Year of publication
1996
Pages
421 - 426
Database
ISI
SICI code
0268-1218(1996)34:6<421:PACOA9>2.0.ZU;2-B
Abstract
A glycoprotein with an apparent molecular weight of 93 kDa was purifie d from a water-soluble extract of Aspergillus fumigatus NCPF 2109 by s ingle step affinity chromatography using the mannose-specific snowdrop (Galanthus nivalis) lectin coupled to agarose. The carbohydrate moiet y contained only mannose and galactose. Partial sequencing of cyanogen bromide fragments of the antigen yielded two sequences, KQNKP and GEI PMKF?PQL, with no homology to any reported proteins. In a preliminary evaluation of its diagnostic potential the 93 kDa antigen was recogniz ed by the sera of four patients with allergic bronchopulmonary aspergi llosis, in addition to a monoclonal antibody raised against a partiall y purified fraction of the A. fumigatus water-soluble extract.