PACAP STIMULATES C-FOS MESSENGER-RNAS IN SMALL-CELL LUNG-CANCER CELLS

Authors
DRAOUI M HIDA T JAKOWLEW S BIRRER M ZIA F MOODY TW
Citation
M. Draoui et al., PACAP STIMULATES C-FOS MESSENGER-RNAS IN SMALL-CELL LUNG-CANCER CELLS, Life sciences, 59(4), 1996, pp. 307-313
Citations number
31
Categorie Soggetti
Biology,"Medicine, Research & Experimental","Pharmacology & Pharmacy
Journal title
Life sciences → ACNP
ISSN journal
00243205
Volume
59
Issue
4
Year of publication
1996
Pages
307 - 313
Database
ISI
SICI code
0024-3205(1996)59:4<307:PSCMIS>2.0.ZU;2-N
Abstract
The effects of PACAP on c-fos mRNA using small cell lung cancer (SCLC) cell was investigated. PACAP-27 (100 nM) increased c-fos mRNA 5-fold using NCI-N417 cells. The increase was concentration dependent with 0. 1 nM PACAP-27 half maximally increasing c-fos mRNA. Also the increase in c-fos mRNA caused by PACAP was time dependent; being maximal after 1 hour and returning to basal values after 4 hours. PACAP-38 but not P ACAP(28-38) increased c-fos mRNA. One uM PACAP(6-38), a PACAP receptor antagonist, inhibited the increase in c-fos mRNA caused by 1 nM PACAP . These data indicate that PACAP stimulates nuclear oncogene expressio n in SCLC cells.