EFFECT OF GLYCOSYLATION ON CLONED ANF-SENSITIVE GUANYLYL CYCLASE

Cloned ANF-sensitive guanylyl cyclase (GC-A) and ANF-sensitive guanyly l cyclase from adrenal cortex differ in their sensitivity to the ANF a nalogs atriopeptin 1 and urodilatin. To test the hypothesis that these differences are due to different glycosylation, we investigated the e ffect of the N-glycosylation inhibitor tunicamycin on GC-A. Tunicamyci n altered the response of GC-A to atriopeptin 1 and urodilatin, wherea s the sensitivity to ANF remained unchanged. These data indicate that agonist specificities of different ANF-sensitive guanylyl cyclases are influenced by carbohydrate moieties.