Pj. Brett et De. Woods, STRUCTURAL AND IMMUNOLOGICAL CHARACTERIZATION OF BURKHOLDERIA-PSEUDOMALLEI O-POLYSACCHARIDE-FLAGELLIN PROTEIN CONJUGATES, Infection and immunity, 64(7), 1996, pp. 2824-2828
The O-polysaccharide moiety of Burkholderia pseudomallei 319a lipopoly
saccharide was covalently linked to flagellin protein isolated from th
e same strain. A glycoconjugate incorporating adipic acid dihydrazide
as a spacer molecule elicited high-titer immunoglobulin G responses to
both the protein and carbohydrate components of the construct. This i
mmunoglobulin G was capable of protecting diabetic rats from challenge
with a heterologous B. pseudomallei strain.