DIAPAUSE-REGULATED PROTEINS IN THE GUT OF PHARATE FIRST INSTAR LARVAEOF THE GYPSY-MOTH, LYMANTRIA-DISPAR, AND THE EFFECT OF KK-42 AND NECKLIGATION ON EXPRESSION

Two groups of proteins in the gut of the gypsy moth, Lymantria dispar, have distinct expression patterns in relation to diapause, Two protei ns, 30 and 35 kDa, are highly expressed prior to the inception of diap ause (13-17 days after oviposition) and then cease to be expressed sho rtly thereafter, Two other proteins, 55 and 60 kDa, are highly express ed in early diapause and then gradually decline in expression as diapa use progresses, Expression of the 55 kDa protein ceases during the chi lling period required to terminate diapause while expression of the 60 kDa protein continues even in postdiapause, Additional experiments fo cused on the 55 kDa protein due to its expression being restricted to early diapause, The 55 kDa protein is gut specific and within the gut it is expressed in only the mid- and hindgut, KK-42, an imidazole deri vative known to avert diapause in the gypsy moth, prevents expression of the 55 kDa protein, Neck ligation experiments indicate that the bra in is not needed to stimulate synthesis of the protein, The protein, h owever, is not synthesized when the gut is cultured iii vitro, This su ggests that some tissue other than the brain and external to the gut i s needed to prompt synthesis of the 55 kDa protein. Copyright (C) 1996 Elsevier Science Ltd