FLUORESCENT FOCUS REDUCTION ASSAY FOR THE SCREENING OF ANTIADENOVIRALAGENTS

Authors
MENTEL R MATTHES E JANTALIPINSKI M WEGNER U
Citation
R. Mentel et al., FLUORESCENT FOCUS REDUCTION ASSAY FOR THE SCREENING OF ANTIADENOVIRALAGENTS, Journal of virological methods, 59(1-2), 1996, pp. 99-104
Citations number
13
Categorie Soggetti
Virology,"Biochemical Research Methods","Biothechnology & Applied Migrobiology
Journal title
Journal of virological methods → ACNP
ISSN journal
01660934
Volume
59
Issue
1-2
Year of publication
1996
Pages
99 - 104
Database
ISI
SICI code
0166-0934(1996)59:1-2<99:FFRAFT>2.0.ZU;2-Q
Abstract
A method for screening of antiviral compounds against adenoviruses was established. Test compounds were diluted and plated in chamber slides for tissue culture. Drug-treated, virus-infected cultures were staine d with fluorescein isothiocyanate conjugated rabbit antibodies against adenovirus hexon type 2 and fluorescent cells were counted by microsc opy. This assay is more sensitive than the colorimetric method and req uires smaller volumes of compounds when compared with the standard met hod using plaque assay.