2-DIMENSIONAL GEL PROTEIN DATABASE OF SACCHAROMYCES-CEREVISIAE

With the systematic sequencing of the yeast genome, yeast biology has entered a new era where novel challenges have to be faced. One challen ge is the identification of the function of the several hundred novel genes discovered by genome sequencing. Another is to understand how al l yeast genes act in concert to ensure and maintain cell organization. Two-dimensional (2-D) gel electrophoresis is the technique of choice to take up these challenges because it provides the opportunity of obt aining an overall view of genome expression. In prospect of these stud ies we have undertaken the construction of a yeast 2-D gel protein dat abase that contains information on polypeptides of the yeast protein m ap. In this paper we report the information presently contained in thi s database. The reported information includes the identification of 25 0 protein spots and the characterization of polypeptides corresponding to N-terminal acetylated proteins, mitochondrial proteins, glucose-re pressed proteins, heat shock induced proteins and proteins encoded by intron-containing genes. In all, 600 spots are annotated. These data c an be accessed on the Yeast Protein Map server through the World Wide Web network.