USE OF 2-DIMENSIONAL GEL-ELECTROPHORESIS TO CHARACTERIZE PROTEIN-SYNTHESIS DURING NEURONAL DEATH IN CEREBELLAR CULTURE

Two-dimensional gel electrophoresis was performed to further investiga te the biochemical changes in protein synthesis observed in two neuron al death models, induced respectively by cytosine arabinoside and glut amate. These drugs induced, respectively, apoptotic and necrotic types of cell death in cerebellar cultures, as previously reported. Most of the proteins showed decreased labeling after toxic exposure, as expec ted, but some polypeptides showed increased labeling or appeared to be newly synthesized. The identification of these polypeptides and their implication in neuronal death are discussed.