A METHOD FOR EXTRACTION OF HIGH-MOLECULAR-WEIGHT DNA FROM THE MACROALGA PORPHYRA-YEZOENSIS (RHODOPHYTA)

Authors
KITADE Y YAMAZAKI S SAGA N
Citation
Y. Kitade et al., A METHOD FOR EXTRACTION OF HIGH-MOLECULAR-WEIGHT DNA FROM THE MACROALGA PORPHYRA-YEZOENSIS (RHODOPHYTA), Journal of phycology, 32(3), 1996, pp. 496-498
Citations number
15
Categorie Soggetti
Plant Sciences","Marine & Freshwater Biology
Journal title
Journal of phycology → ACNP
ISSN journal
00223646
Volume
32
Issue
3
Year of publication
1996
Pages
496 - 498
Database
ISI
SICI code
0022-3646(1996)32:3<496:AMFEOH>2.0.ZU;2-6
Abstract
We developed a method for extraction of DNA from the red alga Porphyra yezoensis Ueda. The method consists of three preparation steps that i nclude CsCl-gradient ultracentrifugation, cetyl trimethyl ammonium bro mide treatment, and a final RNase step. The amount of DNA extracted fr om 1.5 g of starting material averaged 17.7 mu g. The resulting DNA ha d a high molecular weight, was 25-166 kb in length, was digested with five common restriction enzymes, and showed no nuclease activity. It w as of sufficient quality for construction of genomic libraries.