Th. Tsai et al., DETERMINATION OF DICENTRINE IN RAT PLASMA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY AND ITS APPLICATION TO PHARMACOKINETICS, Journal of chromatography B. Biomedical applications, 681(2), 1996, pp. 277-281
Citations number
11
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Journal of chromatography B. Biomedical applications
A simple high-performance liquid chromatographic method was developed
to study the pharmacokinetics of dicentrine in rat plasma after 10 mg/
kg intravenous administration. After addition of an internal standard
(coumarin), plasma was deproteinized by acetonitrile for sample clean-
up. The drugs were separated on a reversed-phase Nucleosil C-18 column
(250 x 4 mm I.D., particle size 5 mu m) and detected by photodiode-ar
ray detection at a wavelength of 308 nm. Acetonitrile-water (35:65, v/
v, pH 2.5-2.8, adjusted with orthophosphoric acid) was used as the mob
ile phase. A biphasic phenomenon with a rapid distribution followed by
a slower elimination phase was observed from the plasma concentration
-time curve.