R. Oconnor et al., DETERMINATION OF SERUM AND TISSUE-LEVELS OF PHENAZINES INCLUDING CLOFAZIMINE, Journal of chromatography B. Biomedical applications, 681(2), 1996, pp. 307-315
Citations number
19
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Journal of chromatography B. Biomedical applications
A rapid and sensitive HPLC method is described for the analysis of syn
thetic phenazines, including clofazimine, from a variety of biological
samples. Phenazines were extracted from serum, tissue and fat using a
mixture of dichloromethane and sodium hydroxide. The drugs were then
quantified on a reversed-phase C-18 column using a mobile phase consis
ting of 594 ml of water, 400 ml of tetrahydrofuran, 6 ml of concentrat
ed acetic acid and 0.471 g of hexanesulfonic acid. In this mobile phas
e, each phenazine tested had its own retention time. This allowed one
phenazine to be used as an internal standard for the analysis of other
phenazines. The method was validated for clofazimine ophenyl)-2,10-di
hydro-2-(isopropylimino)phenazine] and B4090 2-(2,2,6,6-tetramethylpip
erid-4-ylimino)phenazine] (VI) and shown to be accurate and precise ac
ross a broad concentration range from 0.01 to 50 mu g/g (mu g/ml). Ext
raction was 100% for each agent across this range. This system was use
d to measure clofazimine and VI levels following their administration
to rats. The pharmacokinetic profile of VI was different to that of cl
ofazimine, with high tissue concentrations but lower fat levels.