AUTOMATED QUANTITATIVE-DETERMINATION OF A NEW POLYAMINE BIOSYNTHESIS INHIBITOR (CGP-48,664) AND A POTENTIAL METABOLITE IN HUMAN AND ANIMAL PLASMA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

A specific and sensitive liquid chromatographic assay for the determin ation of 4-amidino-1-indanone-2'-amidinohydrazone (CGP 48 664, I) and a potential metabolite, 2-(4-carbamoyl-2,3-dihydro-1H-inden-1-yliden) hydrazine carboximidamide (CGP 53 391, II), in human and animal plasma was developed. CGP 51 467, a structural analog, was added to the plas ma samples (up to 1 ml) as an internal standard. After mixing, the sam ples were processed automatically using an ASPEC solid-phase extractio n system. The final extracts were chromatographed on a 5 mu m Purosphe r RP-18 HPLC column. Chromatography was performed using a gradient sys tem and UV detection. The described HPLC method is suitable for specif ic and quantitative measurement of concentrations of I, as well as its potential metabolite II down to 5-10 ng/ml in human and animal (dog, rat) plasma with acceptable reproducibility and accuracy.