Cp. Chen et Gm. Pollack, DEVELOPMENT OF A CAPILLARY ZONE ELECTROPHORESIS ASSAY TO EXAMINE THE DISPOSITION OF [D-PEN(2,5)]ENKEPHALIN IN RATS, Journal of chromatography B. Biomedical applications, 681(2), 1996, pp. 363-373
Citations number
30
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Journal of chromatography B. Biomedical applications
A novel capillary zone electrophoresis (CZE) assay method was develope
d to evaluate the systemic disposition of [D-pen(2,5)]enkephalin (DPDP
E) in rats. DPDPE was recovered from serum samples (200 mu l) by solid
-phase extraction. Complete resolution of DPDPE and the internal stand
ard ([D-ser(2)]leucine-enkephalin; DSLET) from other serum components
was achieved within 15 min on a 50-mu m I.D. capillary column with ber
ate buffer (25 mM, pH 8.3). The peak-height ratio (DPDPE to DSLET) was
linear through 100 mu g/ml, with a detection limit of 250 ng/ml in se
rum, when absorbance of the column eluent was monitored at 210 nm. Ser
um samples obtained from rats after a 10 mg/kg intravenous bolus dose
of DPDPE were analyzed with the present CZE method. The results sugges
t that CZE is a useful technique for quantitating therapeutic peptides
in biological matrices.