SELECTIVE CYCLODEXTRIN INHIBITION OF ALFAXOLONE-INDUCED ATAXIA

The effect of the use of a number of popular solubility treatments was examined on alfaxolone- and diazepam-induced ataxia. The effects of d iazepam were not significantly altered by solution in cyclodextrin, Al kamuls EL-620 or a mixture of propylene glycol and ethanol. The effect s of alfaxolone were not altered by solution in Alkamuls EL-620, but w ere significantly lessened by solution in cyclodextrin. In a dose-resp onse experiment, the ED50 of alfaxolone increased from 15.3 mg kg(-1) (in Alkamuls EL-620) to 25.6 mg kg(-1) (in hydroxypropyl-beta-cyclodex trin). The results suggest that although cyclodextrins are popular and effective solubilizers, their use must be considered carefully in the context of the experiments in which they are to be used.