PHYSICAL MAPPING OF THE HUMAN NEUROTENSIN GENE (NTS) BETWEEN MARKERS D12S1444 AND D12S81 ON CHROMOSOME 12Q21

Neurotensin (NTS) is an endogenous tridecapeptide of the central nervo us system and the gastrointestinal tract of different mammalian specie s including human. The human gene encoding neurotensin has previously been assigned to chromosome 12 but no regional localization was availa ble. We now confirm this assignment and place the NTS gene on the phys ical and cytogenetic maps. The NTS gene is located on a yeast artifici al chromosome contig that contains several polymorphic markers and is close to a polymorphic marker located at 95.8 cM on the Genethon linka ge map. NTS is immediately proximal to four polymorphic markers, inclu ding D12S81 (AFM102xg9) and D12588 (AFM158yb4). Using fluorescence in situ hybridization, we map the gene cytogenetically to band 12q21. (C) 1996 Academic Press, Inc.