This study shows that carboxylesterase activity is correlated to the d
ifferentiation of primary vascular tissue; it was also observed in epi
dermal cells, located between the outer and inner integuments and in t
he endosperm cells of carrot seed, and in seedlings. Our data support
the idea that the detection of carboxylesterase activity associated wi
th the detection of other enzymes related to secondary wall formation
can be used as a molecular marker for the study of vascular differenti
ation, but cannot be used as an exclusive highly specific marker.