CRYSTALLIZATION AND PRELIMINARY-X-RAY ANALYSIS OF A HYDANTOINASE FROMARTHROBACTER-AURESCENS DSM-3745

Authors
MAY O SIEMANN M SYLDATK C NIEFIND K SCHOMBURG D
Citation
O. May et al., CRYSTALLIZATION AND PRELIMINARY-X-RAY ANALYSIS OF A HYDANTOINASE FROMARTHROBACTER-AURESCENS DSM-3745, Acta crystallographica. Section D, Biological crystallography, 52, 1996, pp. 1209-1210
Citations number
17
Categorie Soggetti
Crystallography,"Biochemical Research Methods",Biology
Journal title
Acta crystallographica. Section D, Biological crystallography → ACNP
ISSN journal
09074449
Volume
52
Year of publication
1996
Part
6
Pages
1209 - 1210
Database
ISI
SICI code
0907-4449(1996)52:<1209:CAPAOA>2.0.ZU;2-0
Abstract
L-Hydantoinase from Arthrobacter aurescens DSM 3745 has been purified to homogeneity and crystallized from polyethylene glycol solutions in a form suitable for X-ray diffraction analysis. The crystals have been grown by the sitting-drop variant of the vapour-diffusion method. X-r ay diffraction studies show that the crystals belong to the monoclinic space group P2(1) with a = 111.2, b = 74.4, c = 146.5 Angstrom and be ta = 106.7 degrees. Its asymmetric unit contains four monomers related by 222 symmetry. The crystals diffract to at least 2.6 Angstrom.