HALISTANOL DISULFATE-B, A NOVEL SULFATED STEROL FROM THE SPONGE PACHASTRELLA SP - INHIBITOR OF ENDOTHELIN-CONVERTING ENZYME

As part of a search for novel inhibitors of endothelin converting enzy me (ECE), the MeOH extract of a South African sponge, Pachastrella sp. , was shown to be active. Bioassay-guided fractionation of the extract yielded a novel sterol sulfate, halistanol disulfate B (1). The struc ture and stereochemistry of 1 was established mainly by interpretation of spectral data. Disulfate (1) was found to be active at a micromola r concentration in the ECE assay.