INVESTIGATION OF THE EFFECT OF ANTINEOPLASTIC DRUGS, CYCLOPHOSPHAMIDE, CISPLATIN AND METHOTREXATE ON THE TURNOVER KINETICS OF HUMAN ERYTHROCYTE ACETYLCHOLINESTERASE

The present work addresses the effects of three antineoplastic drugs [ cyclophosphamide (CP), cisplatin (CDDP) and methotrexate (MTX) which a re in current use for the treatment of various tumors] on turnover kin etics of human erythrocyte membrane-bound acetylcholinesterase (AcChoE ase, EC 3.1.1.7). It was found that CP and MTX significantly decreased k(cat) and k(sp), whereas CDDP decreased only the k(cat) value, (k(sp ) was non-significantly affected). In light of these findings, the CP, CDDP and MTX needs particular attention in tumor therapy and their ef fects on turnover number must be considered at the time of administeri ng these drugs to cancer patients.