EFFECT OF OXYGEN-ENRICHED AERATION ON REGENERATION OF RICE (ORYZA-SATIVA L) CELL-CULTURE

The effect of the oxygen concentration in the aeration gas on regenera tion from rice cells in bioreactor cultures was investigated. The effi ciency of regeneration in cultures aerated with over 40% oxygen was hi gher than that in a flask culture. In the case of a culture in which t he dissolved oxygen(DO) was saturated by aeration with air, the effici ency of regeneration was less than the half that of cultures aerated w ith 40% oxygen. In cultures with the DO levels controlled at 8,10 and 12 mg/ l, the efficiency of regeneration was highest at 12 mg/ l. In t he oxygen-enriched cultures, although cell aggregation was observed an d the color of plantlets was relatively pale, more than 90% of them gr ew into healthy plants.