SEQUENCE-ANALYSIS OF THE ERCC2 GENE REGIONS IN HUMAN, MOUSE, AND HAMSTER REVEALS 3 LINKED GENES

The ERCC2 (excision repair cross-complementing rodent repair group 2) gene product is involved in transcription-coupled repair as an integra l member of the basal transcription factor BTF2/TFIIH complex. Defects in this gene can result in three distinct human disorders, namely the cancer-prone syndrome xeroderma pigmentosum complementation group D, trichothiodystrophy, and Cockayne syndrome. We report the comparative analysis of 91.6 kb of new sequence including 54.3 kb encompassing the human ERCC2 locus, the syntenic region in the mouse (32.6 kb), and a further 4.7 kb of sequence 3' of the previously reported ERCC2 region in the hamster, In addition to ERCC2, our analysis revealed the presen ce of two previously undescribed genes in all three species. The first is centromeric (in the human) to ERCC2 and is most similar to the kin esin light chain gene in sea urchin. The second gene is telomeric (in the human) tee ERCC2 and contains a motif found in ankyrins, some cell cycle proteins, and transcription factors. Multiple EST matches to th is putative new gene indicate that it is expressed in several human ti ssues, including breast. The identification and description of two new genes provides potential candidate genes for disorders mapping to thi s region of 19q13.2. (C) 1996 Academic Press, Inc.