ASSIGNMENT OF THE HYPOXIA-INDUCIBLE FACTOR-1-ALPHA GENE TO A REGION OF CONSERVED SYNTENY ON MOUSE CHROMOSOME-12 AND HUMAN-CHROMOSOME-14Q

Hypoxia-inducible factor 1 (HIF-1) is a basic helix-loop-helix transcr iption factor that mediates homeostatic responses to hypoxia, HIF-1 is a heterodimer consisting of HIF-1 alpha, which is encoded by the HIF1 A gene, complexed with HIF-1 beta, which is encoded by the ARNT gene. In this paper we report the assignment of Hif1a and HIF1A to mouse chr omosome 12 and human chromosome 14, respectively. HIF1A was assigned t o human chromosome 14q21-q24 by analysis of somatic cell hybrids and b y fluorescence in situ hybridization. Hif1a was localized by interspec ific backcross analysis within a region of mouse chromosome 12 encompa ssing >30 cM that demonstrates conservation of synteny with a region o f human chromosome 14 extending from PAX9 at 14q12-q13 to IGHC at 14q3 2.33. (C) 1996 Academic Press, Inc.