NADH-FERREDOXIN REDUCTASE AND NAD-REDUCING HYDROGENASE ACTIVITIES IN HYDROGENOBACTER-THERMOPHILUS STRAIN-TK-6

Authors
YOON KS UEDA Y ISHII M IGARASHI Y KODAMA T
Citation
Ks. Yoon et al., NADH-FERREDOXIN REDUCTASE AND NAD-REDUCING HYDROGENASE ACTIVITIES IN HYDROGENOBACTER-THERMOPHILUS STRAIN-TK-6, FEMS microbiology letters, 139(2-3), 1996, pp. 139-142
Citations number
12
Categorie Soggetti
Microbiology
Journal title
FEMS microbiology letters → ACNP
ISSN journal
03781097
Volume
139
Issue
2-3
Year of publication
1996
Pages
139 - 142
Database
ISI
SICI code
0378-1097(1996)139:2-3<139:NRANHA>2.0.ZU;2-M
Abstract
NADH:ferredoxin reductase (EC 1.18.1.3) and NAD-reducing hydrogenase ( EC 1.12.1.2) activities were detected in the cytoplasm of Hydrogenobac ter thermophilus TK-6. NADH:ferredoxin reductase activity was detected using metronidazole, an artificial electron acceptor, which reacts sp ecifically with reduced ferredoxin. Soluble NAD-reducing hydrogenase a ctivity was detected after extended preincubation. The lag disappeared when cell-free extract was incubated anaerobically for more than 30 m in. The electron transport system of this chemolithoautotrophic bacter ium is discussed.