Y. Yasuda et al., IMMUNOLOGICAL DETECTION OF THE GERA SPORE GERMINATION PROTEINS IN THESPORE INTEGUMENTS OF BACILLUS-SUBTILIS USING SCANNING ELECTRON-MICROSCOPY, FEMS microbiology letters, 139(2-3), 1996, pp. 235-238
To clarify the molecular mechanisms that trigger spore germination of
Bacillus subtilis, the location of GerA proteins (GerAA, GerAB and Ger
AC), which were reported to be putative gene products of a receptor fo
r one of the germinants, L-alanine, was investigated by immunological
techniques using anti-GerA peptide antibodies. Four antibodies were ra
ised against the corresponding epitopes, two in GerAA, one in GerAB an
d the other in GerAC molecules, The binding of all four antibodies to
the inner surface of the cortex-less spore coat fragments could be see
n by scanning immunoelectron microscopy with colloidal gold particles.
The result agreed with the fact, previously reported, that the colloi
dal gold particles were visualized just inside the spore coat layer by
transmission immunoelectron microscopy using another anti-GerAB pepti
de antibody.