PROTEASE ACTIVATED RECEPTORS MODULATE AORTIC VASCULAR TONE

The effect of agonists of the known protease activated receptors (PAR) , the thrombin and the PAR-2 receptors, on vasoactive mediator release and vascular tone were studied using rings of rat aorta. Stimulation of aortic rings with the thrombin receptor agonist, Trap-14 or the PAR -2 agonist, SLIGRL, resulted in a rapid release of nitric oxide. Trap- 14 and SLIGRL-induced nitric oxide release was reduced by pre-treatmen t with BQ-788, an ET(B) endothelin receptor-specific antagonist, Consi stent with a role for endothelin-1 receptor activation in Trap-14 and SLIGRL-induced nitric oxide release, endothelin-1 levels were increase d significantly following 5 min treatment of aortic rings with Trap-14 or SLIGRL. Cumulative addition of Trap-14 to aortic rings denuded of endothelium resulted in dose-dependent contraction with an EC(50) valu e of 23 +/- 5 mu M, whereas SLIGRL addition failed to induce aortic co ntraction. These data suggest that the known protease activated recept ors are functionally coupled to nitric oxide release. In addition, the thrombin receptor appears to modulate both vasodilator and contractil e responses, whereas the PAR-2 receptor is linked only to vasodilation .