THE ACTIVITY OF THE HIGH-AFFINITY K-COLI TO METHYLGLYOXAL( UPTAKE SYSTEM KDP SENSITIZES CELLS OF ESCHERICHIA)

Authors
FERGUSON GP CHACKO AD LEE C BOOTH IR
Citation
Gp. Ferguson et al., THE ACTIVITY OF THE HIGH-AFFINITY K-COLI TO METHYLGLYOXAL( UPTAKE SYSTEM KDP SENSITIZES CELLS OF ESCHERICHIA), Journal of bacteriology, 178(13), 1996, pp. 3957-3961
Citations number
30
Categorie Soggetti
Microbiology
Journal title
Journal of bacteriology → ACNP
ISSN journal
00219193
Volume
178
Issue
13
Year of publication
1996
Pages
3957 - 3961
Database
ISI
SICI code
0021-9193(1996)178:13<3957:TAOTHK>2.0.ZU;2-Q
Abstract
Expression of the Kdp system sensitizes cells to methylglyoxal (MG) wh ether this electrophile is added externally or is synthesized endogeno usly. The basis of this enhanced sensitivity is the maintenance of a h igher cytoplasmic pH (pHi) in cells expressing Kdp. In such cells, MG elicits rapid cytoplasmic acidification via KefB and KefC, but the ste ady-state pHi attained is still too high to confer protection. Lowerin g pHi further by incubation with acetate decreases the sensitivity of cells to MG.