PROTEINS INVOLVED IN THE VROMAN EFFECT DURING EXPOSURE OF HUMAN BLOOD-PLASMA TO GLASS AND POLYETHYLENE

The amounts of fibrinogen adsorbed to glass from various human blood p lasmas have been measured as a function of time. The plasmas were 11 s ingle donor plasmas, pooled plasma, a single donor high molecular weig ht kininogen (HMWK)-deficient plasma and HMWK-deficient plasma, which had been reconstituted with HMWK. For adsorption times between 1 min a nd 1 h more fibrinogen adsorbed from HMWK-deficient plasma compared wi th the amounts of fibrinogen which adsorbed from the other plasmas. Th is result supports the conclusion of several authors that HMWK is invo lved in the displacement of fibrinogen, initially adsorbed from normal human plasma to glass. Class surfaces, preexposed to solutions of pla sma and subsequently exposed to 1:1 diluted plasma, gives rise to a re latively high adsorption of HMWK which is independent of the plasma co ncentration of the precoating solution. The results indicate that HMWK from 1:1 diluted plasma is involved in the displacement of proteins f rom glass surfaces which had been pre-exposed to solutions with a low plasma concentration. Experiments with polyethylene as a substrate rev eal that high density lipoprotein (HDL) from 1:1 diluted plasma is inv olved in the displacement of proteins from polyethylene surfaces which had been pre-exposed to solutions with a low plasma concentration. Mo reover, evidence is presented that substantial amounts of albumin and fibrinogen, adsorbed from 1:1000 diluted plasma to glass and polyethyl ene, are displaced from the surfaces of these materials by proteins fr om 1:1 diluted plasma different from HMWK and HDL. Copyright (C) 1996 Elsevier Science Limited