D. Despeyroux et al., ELECTROSPRAY MASS-SPECTROMETRY FOR DETECTION AND CHARACTERIZATION OF PURIFIED CRICKET PARALYSIS VIRUS (CRPV), Rapid communications in mass spectrometry, 10(8), 1996, pp. 937-941
Electrospray mass spectrometry has been used to detect and characteriz
e the capsid proteins of a non-disrupted sample of cricket paralysis v
irus. By direct injection and by on-line liquid chromatography/mass sp
ectrometry of the intact virus, three capsid proteins (VP1, VP2 and VP
3) were detected and their relative molecular masses (RMM) accurately
determined. In addition to these proteins, a fourth polypeptide (RMM=6
012 Da) was detected which, by analogy with mammalian picornaviruses,
may correspond to the capsid protein VP4. The finding of this latter p
rotein now reduces the uncertainty of the classification of CrPV as a
picornavirus.