SPECTROPHOTOMETRIC ASSAY FOR PROCESSES INVOLVING CHANGES IN HYDROGEN-ION CONCENTRATION IN AQUEOUS-SOLUTION

Authors
PENKETH PG SHYAM K PATTON CL SARTORELLI AC
Citation
Pg. Penketh et al., SPECTROPHOTOMETRIC ASSAY FOR PROCESSES INVOLVING CHANGES IN HYDROGEN-ION CONCENTRATION IN AQUEOUS-SOLUTION, Analytical biochemistry, 238(1), 1996, pp. 46-49
Citations number
13
Categorie Soggetti
Biology
Journal title
Analytical biochemistry → ACNP
ISSN journal
00032697
Volume
238
Issue
1
Year of publication
1996
Pages
46 - 49
Database
ISI
SICI code
0003-2697(1996)238:1<46:SAFPIC>2.0.ZU;2-S
Abstract
A strategy for the design of simple colorimetric assays to follow any process that involves the net generation or consumption of hydrogen io ns in aqueous solutions over the pH range of 3-10 is described. This p rocedure relies upon the measurement of the change in absorption when a weakly or moderately buffered solution of a pH indicator is subjecte d to a small change in pH. Buffers and indicators are chosen with clos ely matching pK(a) values. The versatility of this type of assay techn ique is illustrated using three examples. (C) 1996 Academic Press, Inc .