FLUORESCENCE IN-SITU HYBRIDIZATION - A BRIEF REVIEW

Fluorescence in situ hybridization (FISH) is used for many purposes, i ncluding analysis of chromosomal damage, gene mapping, clinical diagno stics, molecular toxicology and cross-species chromosome homology. FIS H allows an investigator to identify the presence and location of a re gion of cellular DNA or RNA within morphologically preserved chromosom e preparations, fixed cells or tissue sections. This report describes in situ hybridization, and discusses the post, present and future appl ications of this method for genetic analysis and molecular toxicology. (C) 1996 Wiley-Liss, Inc.