INFLUENCE OF CHOLESTEROL AND PROTEIN-DIET ON LIVER CYTOCHROME P-450-DEPENDENT MONOOXYGENASE SYSTEM IN RATS

The influence of the cholesterol and protein diet on the mixed-functio n oxidases system activity and desaturation of fatty acids were examin ed. The phenobarbital innducibility of these parameters was analyzed t oo. Investigations were carried out on Wistar adult male rats. The ani mals were on the cholesterol(0.25%) and protein diet (32%) during 45 d ays. The cytochrome P-450 and cytochrome b(5) contents, the NADPH-cyto chrome P-450 reductase and NADH-cytochrome b(5) reductase activities, aniline hydroxylase and 4-aminopyrine N-demethylase activities were me asured in the hepatic microsomal fraction. In both sexes, the choleste rol diet decreased all the examined parameters with the exception of N ADH-cytochrome b, reductase activity. The protein diet did not change the examined enzyme activities or levels with the exception of inducti on of NADH-cytochrome b(5) reductase and 4-aminopyrine N-demethylase a ctivities. Simultaneous treatment with phenobarbital had heterogenous effect dependent on the type of diet and examined parameter.