B. Christensen et al., TREATMENT OF ACID-MINE WATER BY SULFATE-REDUCING BACTERIA - RESULTS FROM A BENCH-SCALE EXPERIMENT, Water research, 30(7), 1996, pp. 1617-1624
Overflowing water from waterfilled mines and open pits in pyrite and c
oal mine areas is often acid and severely polluted by metals and sulfa
te. The effect of stimulating bacterial sulfate reduction in such syst
ems in order to improve water quality was examined in a bench scale ex
periment. Polyacrylate cylinders with a sand bed and a layer of crushe
d stones at the bottom were filled with acid mine water, supplied with
whey to support bacterial growth and incubated for 203 d. After an in
itial lag phase, pH started to increase, redox potentials dropped and
dissolved concentrations of copper, zinc, iron and aluminium were redu
ced in cylinders supplied with whey. Simultaneously, a black sludge wa
s formed. Acetate tended to accumulate as an undesired waste product.
The observations indicate that sulfate-reducing bacteria (SRB) became
active in the cylinders and contributed to metal removal. Inoculation
with SRB shortened the initial lag phase, but was not necessary to ini
tiate the sulfate-reduction process. The in situ treatment concept may
be useful in relatively small and well defined mine water systems, wh
ere the process can be governed properly. (C) 1996 Elsevier Science Lt
d