AMELIORATION OF HEPATOCELLULAR INTEGRITY AND INHIBITION OF SINUSOIDALOXIDATIVE STRESS BY N-ACETYLCYSTEINE PRETREATMENT IN COLD ISCHEMIA-REPERFUSION INJURY OF RAT-LIVER

Further improvements of donor liver preservation are still required in liver transplantation. In the present study, we investigated whether intraportal injection of N-acetylcysteine (NAC) 15 min before flush-ou t of UW solution (NAC pre treatment) improves liver dysfunction after cold preservation or has a protective effect on sinusoidal oxidative s tress. The effect of NAC pretreatment was examined using an isolated p erfused rat liver model. The NAC pretreatment significantly reduced si nusoidal oxidative stress relative to a control dextrose 5% injection. Under a glutathione-depleted condition produced by L-buthionine-[S-R] -sulfoximine, the NAC pre treatment also significantly reduced hepatoc ellular as well as sinusoidal oxidative stress, resulting in improveme nt of hepatocelllar integrity relative to a control dextrose 5% inject ion.