BLEND MEMBRANE OF REGENERATED SILK FIBROIN, POLY(VINYL ALCOHOL), AND PEROXIDASE AND ITS APPLICATION TO A FERROCENE-MEDIATING HYDROGEN-PEROXIDE SENSOR

Before or after the blend membrane of regenerated silk fibroin (RSF), poly(vinyl alcohol) (PVA), and peroxidase is treated with ethanol, RSF , PVA, and peroxidase maintain their own structures. The conformationa l transition of RSF in the blend membrane is accomplished from the sil k I structure to the silk II structure by ethanol treatment, which is used to immobilize peroxidase. A ferrocene-mediating sensor for H2O2 i s made, which is based on the immobilization of peroxidase in the blen d membrane of RSF and PVA. Performance and characteristics of the sens or were evaluated with respect to response time, detection limit, sele ctivity, and dependencies on temperature and pH as well as on operatin g and storage stability. (C) 1996 John Wiley & Sons, Inc.