DIFFERENTIAL ENDOTHELIAL MIGRATION AND PROLIFERATION TO BASIC FIBROBLAST GROWTH-FACTOR AND VASCULAR ENDOTHELIAL GROWTH-FACTOR

Citation
A. Yoshida et al., DIFFERENTIAL ENDOTHELIAL MIGRATION AND PROLIFERATION TO BASIC FIBROBLAST GROWTH-FACTOR AND VASCULAR ENDOTHELIAL GROWTH-FACTOR, Growth factors, 13(1-2), 1996, pp. 57-64
Citations number
33
Categorie Soggetti
Biology,"Cell Biology
Journal title
ISSN journal
08977194
Volume
13
Issue
1-2
Year of publication
1996
Pages
57 - 64
Database
ISI
SICI code
0897-7194(1996)13:1-2<57:DEMAPT>2.0.ZU;2-J
Abstract
Neovascularization is a feature of a variety of pathological processes . We compared the characteristics of vascular endothelial growth facto r (VEGF) and basic fibroblast growth factor (bFGF) on migration and pr oliferation of human umbilical vein endothelium (HUVEC). Both VEGF and bFGF induced endothelial cell migration at similar concentrations (1/ 2 max. VEGF = similar to 1.0 ng/ml, bFGF = similar to 5.0 ng/ml). Howe ver, VEGF-stimulated migration was two-fold greater than bFGF at 1 and 10 ng/ml (rho < 0.05). In contrast, bFGF induced proliferation four-f old more effectively than VEGF (1/2 max. 1 ng/ml and 1.4 ng/ml respect ively). Checkerboard migration assays for bFGF showed a predominantly chemokinetic pattern, whereas VEGF was predominantly chemotactic. VEGF and bFGF were not synergistic in monolayer proliferation and migratio n assays. Three angiogenesis inhibitors, alpha-interferon, TNP-470, an d platelet factor-4, inhibited VEGF and bFGF induced cell migration. T hese results indicate that VEGF and bFGF are chemoattractants that sti mulate endothelial migration by different mechanisms and that both can be inhibited by known angiogenesis inhibitors.