Bs. Deshpande et al., CEPHALOSPORIN-C ACYLASE AND PENICILLIN-V ACYLASE FORMATION BY AEROMONAS SP ACY-95, World journal of microbiology & biotechnology, 12(4), 1996, pp. 373-378
Aeromonas sp. ACY 95 produces constitutively and intracellularly a pen
icillin V acylase at an early stage of fermentation (12 h) and a cepha
losporin C acylase at a later stage (36 h). Some penicillins, cephalos
porin C and their side chain moieties/analogues, phenoxyacetic acid, p
enicillin V and penicillin G, enhanced penicillin V acylase production
while none of the test compounds affected cephalosporin C acylase pro
duction. Supplementation of the medium with some sugars and sugar deri
vatives repressed enzyme production to varying degrees, The studies on
enzyme formation, induction and repression, and substrate profile sug
gest that the cephalosporin C acylase and penicillin V acylase are two
distinct enzymes. Substrate specificity studies indicate that the Aer
omonas sp. ACY 95 produces a true cephalosporin C acylase which unlike
the enzymes reported hitherto hydrolyses cephalosporin C specifically
.