ANALYSIS OF THE DNA-SEQUENCE OF A 15,500 BP FRAGMENT NEAR THE LEFT TELOMERE OF CHROMOSOME-XV FROM SACCHAROMYCES-CEREVISIAE REVEALS A PUTATIVE SUGAR TRANSPORTER, A CARBOXYPEPTIDASE HOMOLOG AND 2 NEW OPEN READING FRAMES

We report the sequence of a 15.5 kb DNA segment located near the left telomere of chromosome XV of Saccharomyces cerevisiae. The sequence co ntains nine open reading frames (ORFs) longer than 300 bp. Three of th em are internal to other ones. One corresponds to the gene LGT3 that e ncodes a putative sugar transporter. Three adjacent ORFs were separate d by two stop codons in frame. These ORFs presented homology with the gene CPS1 that encodes carboxypeptidase S. The stop codons were not fo und in the same sequence derived from another yeast strain. Two other ORFs without significant homology in databases were also found. One of them, O0420, is very rich in serine and threonine and presents a seri es of repeated or similar amino acid stretches along the sequence. The nucleotide sequence has been deposited in the EMBL data library under Accession Number X89715.