AN ANTI-CD3-EPSILON SERUM DETECTS T-LYMPHOCYTES IN PARAFFIN-EMBEDDED PATHOLOGICAL TISSUES IN MANY ANIMAL SPECIES

The in situ identification of T cells has been difficult and restricte d to frozen tissue samples of a limited range of species. In this stud y, we demonstrate that an antiserum recognizing a phylogenetically con served part of the CD3 epsilon cytoplasmic tail detects T cells of man y avian and mammalian species in formalin-fixed paraffin-embedded tiss ue sections. This antiserum enables the morphological characterization of normal and pathological lymphoid tissues and lymphoid infiltration s in experimental work and in animal disease.