CALCIUM-CARBONATE CRYSTALS PROMOTE CALCIUM-OXALATE CRYSTALLIZATION BYHETEROGENEOUS OR EPITAXIAL NUCLEATION - POSSIBLE INVOLVEMENT IN THE CONTROL OF URINARY LITHOGENESIS
S. Geider et al., CALCIUM-CARBONATE CRYSTALS PROMOTE CALCIUM-OXALATE CRYSTALLIZATION BYHETEROGENEOUS OR EPITAXIAL NUCLEATION - POSSIBLE INVOLVEMENT IN THE CONTROL OF URINARY LITHOGENESIS, Calcified tissue international, 59(1), 1996, pp. 33-37
A large proportion of urinary stones have calcium oxalate (CaOx) as th
e major mineral phase. In these stones, CaOx is generally associated w
ith minor amounts of other calcium salts. Several reports showing the
presence of calcium carbonate (CaCO3) and calcium phosphate in renal s
tones suggested that crystals of those salts might be present in the e
arly steps of stone formation. Such crystals might therefore promote C
aOx crystallization from supersaturated urine by providing an appropri
ate substrate for heterogeneous nucleation. That possibility was inves
tigated by seeding a metastable solution of Ca-45 oxalate with vaterit
e or calcite crystallites. Accretion of CaOx was monitored by Ca-45 in
corporation. We showed that (1) seeds of vaterite (the hexagonal polym
orph of CaCO3) and calcite (the rhomboedric form) could initiate calci
um oxalate crystal growth; (2) in the presence of lithostathine, an in
hibitor of CaCO3 crystal growth, such accretion was not observed. In a
ddition, scanning electron microscopy demonstrated that growth occurre
d by epitaxy onto calcite seeds whereas no special orientation was obs
erved onto vaterite. It was concluded that calcium carbonate crystals
promote crystallization of calcium oxalate and that inhibitors control
ling calcium carbonate crystal formation in Henle's loop might play an
important role in the prevention of calcium oxalate stone formation.