APPLICATION OF LONG-DISTANCE PCR TO RESTRICTION SITE MAPPING OF A CLONED DNA FRAGMENT ON THE LAMBDA-EMBL3 PHAGE VECTOR

Long-distance PCR was applied to rapidly map the restriction sites of long inserts cloned on lambda EMBL3 phage vector. The restriction site s of 9 of 15 enzymes were completely assigned in a model experiment wi thin 14 h, including 8 h for the PCR amplification. This method was fo und particularly useful for genomic DNA cloning when the partial seque nce of the corresponding cDNA is known.