ELECTRIC-FIELD FUSION OF LACTOBACILLUS-PLANTARUM

Authors
SAKANAKA K HIMEJIMA M SHIRAKAWA N
Citation
K. Sakanaka et al., ELECTRIC-FIELD FUSION OF LACTOBACILLUS-PLANTARUM, Bioscience, biotechnology, and biochemistry, 60(6), 1996, pp. 1023-1024
Citations number
14
Categorie Soggetti
Biology,Agriculture,"Biothechnology & Applied Migrobiology","Food Science & Tenology
Journal title
Bioscience, biotechnology, and biochemistry → ACNP
ISSN journal
09168451
Volume
60
Issue
6
Year of publication
1996
Pages
1023 - 1024
Database
ISI
SICI code
0916-8451(1996)60:6<1023:EFOL>2.0.ZU;2-C
Abstract
Protoplast fusion of Lactobacillus plantarum ATCC10012 was examined wi th an electric field fusion method using its double mutants having dru g resistance and auxotrophic marker genes. Under the conditions of pul se width of 150 mu s and an electric field intensity of 6 kV/cm, the f requency of obtained strains carrying the phenotypes from both mutants was highest (6.2 x 10(-7)). The frequency was at least one order high er than the reversion frequencies and that of conjugation. These resul ts suggest the completion of protoplast fusion.