Sq. Shu et al., SEX-PHEROMONE PRODUCTION IN CALLOSOBRUCHUS-MACULATUS (COLEOPTERA, BRUCHIDAE) - ELECTROANTENNOGRAPHIC AND BEHAVIORAL-RESPONSES, Journal of Stored Products Research, 32(1), 1996, pp. 21-30
Behavioral and electroantennogram (EAG) assays were used to determine
rhythmicity and dynamics of the sex pheromone biology of the bruchid b
eetle, Callosobruchus maculatus (F,), Beetles were sexually active thr
oughout the photophase. Virgin females entrained to a 16:8 L:D light c
ycle emitted more sex pheromone during the first half of photophase th
an during the second half of photophase or during scotophase. Sex pher
omone production increased from day 0 to day 3 of adult life, and then
decreased as females grew older, Availability of garbonzo beans, one
of the larval food and adult oviposition hosts, to females did not sti
mulate sex pheromone production. Mating resulted in a reduction in pos
t-coital sex pheromone production. Mated females, however, regained at
tractiveness to males and this attractiveness increased from day 1 to
day 3 after first mating when females had been mated at day 0 after em
ergence. (Z)-3-Methyl-2-heptenoic acid (Z32A) and (Z)-3-methyl-3-hepte
noic acid (Z33A), two compounds implicated as sex pheromone components
for C. maculatus, both elicited male EAG and behavioral responses in
the beetles; however, Z33A elicited larger responses than Z32A. Binary
mixtures of the two compounds were more effective than either compoun
d alone. Copyright (C) 1996 Elsevier Science Ltd