MAPPING OF HUMAN NONMUSCLE TYPE COFILIN (CFL1) TO CHROMOSOME-11Q13 AND MUSCLE-TYPE COFILIN (CFL2) TO CHROMOSOME-14

Cofilin is a widely-distributed, intracellular, actin binding protein which is involved in the translocation of actin-cofilin complex from c ytoplasm to nucleus. We have cloned a non-muscle-type cofilin (CFL1) f rom a human promyelocytic cDNB library and mapped this to human chromo some 11 by PCR amplification of 3' untranslated sequence in a panel of rodent-human somatic cell hybrids, and to the interval 11q12-q13.2 in a chromosome 11 somatic cell hybrid mapping panel. Confirmation of re gional localisation to 11q13 has been obtained by fluorescent in situ hybridisation of genomic cosmid clones, by demonstration of the presen ce of both SEA (the human homologue of avian retrovirus proviral tyros ine kinase, 11q13) and CFL1 in some of these clones and by close linka ge of CFL1 to SEA in a panel of high-dose irradiation hybrids. We have identified human muscle-type cofilin sequences by comparison of human expressed sequence tags with M-type cofilins of other species and we have mapped the human M-type cofilin, CFL2, to chromosome 14.